Recombinant human ASS1 protein

The ASS1 is an enzyme called argininosuccinate synthase 1. This enzyme participates in the urea cycle, which is a sequence of chemical reactions that takes place in liver cells. The urea cycle processes excess nitrogen that is generated as the body uses proteins. The excess nitrogen is used to make a compound called urea, which is excreted from the body in urine. Recombinant human ASS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Argininosuccinate synthase, ASS, CTLN1, Argininosuccinate synthase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

48.6 kDa (432aa) confirmed by MALDI-TOF

Additionnal Information

ASS1, Argininosuccinate synthase, ASS, CTLN1, Argininosuccinate synthase, ATGP0636-10 µg, ATGP0636-20 µg, ATGP0636-50 µg, ATGP0636-100 µg, ATGP0636-250 µg, ATGP0636-500 µg, ATGP0636-1 mg, ATGP0636-10, ATGP0636-20, ATGP0636-50, ATGP0636-100, ATGP0636-250, ATGP0636-500, ATGP0636-1

References & Citations

Kobayashi K, . et al. (1990) J Biol Chem. 265 (19) :11361-7

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000041

Uniprot Accession Number

P00966

Species

Human

AA Sequence

MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK ALKLGAKKVF IEDVSREFVE EFIWPAIQSS ALYEDRYLLG TSLARPCIAR KQVEIAQREG AKYVSHGATG KGNDQVRFEL SCYSLAPQIK VIAPWRMPEF YNRFKGRNDL MEYAKQHGIP IPVTPKNPWS MDENLMHISY EAGILENPKN QAPPGLYTKT QDPAKAPNTP DILEIEFKKG VPVKVTNVKD GTTHQTSLEL FMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIAK SQERVEGKVQ VSVLKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPTDATG FININSLRLK EYHRLQSKVT AK