Recombinant human NUDT5 protein

NuDT5 is a member of the nudix hydrolase family which eliminate toxic nucleotide derivatives from the cell. NuDT5 hydrolyzes ADP-ribose and ADP-mannose in the presence of magnesium, and also hydrolyzes other nucleotide sugars with low activity such as ADP-glucose and diadenosine diphosphate. As a nudix hydrolase, NuDT5 contains a central nudix motif and functions to eliminate toxic nucleotide metabolites from the cell while maintaining the levels of signaling nucleotides. NuDT5 is widely expressed but is most abundant in liver as a homodimer. Recombinant human NuDT5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nudix hydrolase 5, ADP-sugar pyrophosphatase, 8-oxo-dGDP phosphatase, Nuclear ATP-synthesis protein NUDIX5, Nucleoside diphosphate-linked moiety X motif 5, Nudix motif 5, YSA1H, hYSAH1, NUDIX5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

26.5 kDa (239aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will be appear higher)

Additionnal Information

ADP-sugar Pyrophosphatase, NUDT5, ADP-sugar pyrophosphatase, hYSAH1, YSA1, YSA1H, ADP-sugar pyrophosphatase, HSPC115, Nudix motif 5, NuDT-5, NuDT 5, ATGP0633-10 µg, ATGP0633-20 µg, ATGP0633-50 µg, ATGP0633-100 µg, ATGP0633-250 µg, ATGP0633-500 µg, ATGP0633-1 mg, ATGP0633-10, ATGP0633-20, ATGP0633-50, ATGP0633-100, ATGP0633-250, ATGP0633-500, ATGP0633-1

References & Citations

Yu HN., et al. (2007) Biochem Biophys Res Commun. 354 (3) :764-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_054861

Uniprot Accession Number

Q9UKK9

Species

Human

AA Sequence

MESQEPTESS QNGKQYIISE ELISEGKWVK LEKTTYMDPT GKTRTWESVK RTTRKEQTAD GVAVIPVLQR TLHYECIVLV KQFRPPMGGY CIEFPAGLID DGETPEAAAL RELEEETGYK GDIAECSPAV CMDPGLSNCT IHIVTVTING DDAENARPKP KPGDGEFVEV ISLPKNDLLQ RLDALVAEEH LTVDARVYSY ALALKHANAK PFEVPFLKF