Recombinant human WARS protein

WARS, also known as tryptophanyl-tRNA synthetase, belongs to the class I tRNA synthetase family. Two forms of tryptophanyl tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. WARS catalyzes the aminoacylation of tRNA (trp) with tryptophan and is induced by interferon. It also regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Recombinant human WARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tryptophanyl-tRNA synthetase cytoplasmic, GAMMA-2, IFI53, IFP53, Tryptophanyl-tRNA synthetase, cytoplasmic, TrpRS, hWRS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

55.3 kDa (491aa) confirmed by MALDI-TOF

Additionnal Information

WARS, Tryptophanyl-tRNA synthetase cytoplasmic, GAMMA-2, IFI53, IFP53, Tryptophanyl-tRNA synthetase, cytoplasmic, TrpRS, hWRS, ATGP0627-10 µg, ATGP0627-20 µg, ATGP0627-50 µg, ATGP0627-100 µg, ATGP0627-250 µg, ATGP0627-500 µg, ATGP0627-1 mg, ATGP0627-10, ATGP0627-20, ATGP0627-50, ATGP0627-100, ATGP0627-250, ATGP0627-500, ATGP0627-1

References & Citations

Nagano K., et al. (2004) Oncogene. 23 (9) :1693-703.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Scientific Category

Angiogenesis

NCBI Accession Number

NP_776049

Uniprot Accession Number

P23381

Species

Human

AA Sequence

MPNSEPASLL ELFNSIATQG ELVRSLKAGN ASKDEIDSAV KMLVSLKMSY KAAAGEDYKA DCPPGNPAPT SNHGPDATEA EEDFVDPWTV QTSSAKGIDY DKLIVRFGSS KIDKELINRI ERATGQRPHH FLRRGIFFSH RDMNQVLDAY ENKKPFYLYT GRGPSSEAMH VGHLIPFIFT KWLQDVFNVP LVIQMTDDEK YLWKDLTLDQ AYSYAVENAK DIIACGFDIN KTFIFSDLDY MGMSSGFYKN VVKIQKHVTF NQVKGIFGFT DSDCIGKISF PAIQAAPSFS NSFPQIFRDR TDIQCLIPCA IDQDPYFRMT RDVAPRIGYP KPALLHSTFF PALQGAQTKM SASDPNSSIF LTDTAKQIKT KVNKHAFSGG RDTIEEHRQF GGNCDVDVSF MYLTFFLEDD DKLEQIRKDY TSGAMLTGEL KKALIEVLQP LIAEHQARRK EVTDEIVKEF MTPRKLSFDF Q