Recombinant human Creatine kinase B/CKB protein

CKB (Creatine kinase B-type) is a cytoplasmic enzyme involved in energy homeostasis. The protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, tumor and cancer cells. Recombinant human CKB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography technique

Product Specifications

Product Name Alternative

Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, CPK-B, B-CK, CKBB

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

44.8 kDa (401aa) confirmed by MALDI-TOF

Additionnal Information

Creatine Kinase BB, CKB, Creatine kinase B-type, B-CK, CKBB, Creatine kinase B-type, BCK, ATGP0626-10 µg, ATGP0626-20 µg, ATGP0626-50 µg, ATGP0626-100 µg, ATGP0626-250 µg, ATGP0626-500 µg, ATGP0626-1 mg, ATGP0626-10, ATGP0626-20, ATGP0626-50, ATGP0626-100, ATGP0626-250, ATGP0626-500, ATGP0626-1

References & Citations

Chang EJ., et al. (2008) Nat Med. 14 (9) :966-72. ; ; Debrincat MA., et al. (2007) J Biol Chem. 282 (7) :4728-37.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_001814

Uniprot Accession Number

P12277

Species

Human

AA Sequence

MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K