Recombinant human FKBPL protein

FKBPL has similarity to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is thought to have a potential role in the induced radioresistance and it appears to have some involvement in the control of the cell cycle. Also, FKBPL is involved in cellular response to stress. And it is known to interact with Hsp90, glucocorticoid receptor and dynamitin and may play a role in signalling, like other FKBPs. Recombinant FKBPL protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Specifications

Specific activity is > 210 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.

Product Name Alternative

DIR1, FK506 binding protein like, FKBP prolyl isomerase like, NG7, WAF-1/CIP1 stabilizing protein 39, WISP39

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 210 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.

Molecular Weight

39.2 kDa (357aa) confirmed by MALDI-TOF

Additionnal Information

DIR 1, DIR1, DIR-1, FK506 binding protein like, FKBP prolyl isomerase like, FKBPL, NG 7, NG7, NG-7, WAF-1/CIP1 stabilizing protein 39, WISP 39, WISP39, WISP-39, ATGP0623-10 µg, ATGP0623-20 µg, ATGP0623-50 µg, ATGP0623-100 µg, ATGP0623-250 µg, ATGP0623-500 µg, ATGP0623-1 mg, ATGP0623-010, ATGP0623-020, ATGP0623-050, ATGP0623-100, ATGP0623-250, ATGP0623-500, ATGP0623-01M

References & Citations

McKeen HD., et al. (2008) Endocrinology 149: 5724.; ; Jascur T., et al (2005) Mol. Cell 17 (2) : 237-49.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100 mM NaCl

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_071393

Uniprot Accession Number

Q9UIM3

Species

Human

AA Sequence

METPPVNTIG EKDTSQPQQE WEKNLRENLD SVIQIRQQPR DPPTETLELE VSPDPASQIL EHTQGAEKLV AELEGDSHKS HGSTSQMPEA LQASDLWYCP DGSFVKKIVI RGHGLDKPKL GSCCRVLALG FPFGSGPPEG WTELTMGVGP WREETWGELI EKCLESMCQG EEAELQLPGH SGPPVRLTLA SFTQGRDSWE LETSEKEALA REERARGTEL FRAGNPEGAA RCYGRALRLL LTLPPPGPPE RTVLHANLAA CQLLLGQPQL AAQSCDRVLE REPGHLKALY RRGVAQAALG NLEKATADLK KVLAIDPKNR AAQEELGKVV IQGKNQDAGL AQGLRKMFGL EHHHHHH