Recombinant human GCDH protein

Glutaryl-CoA dehydrogenase (GCDH) belongs to the acyl-CoA dehydrogenase family. GCDH catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. This Protein uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. Recombinant GCDH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glutaryl-CoA dehydrogenase, ACAD5, GCD, Glutaryl-CoA dehydrogenase, ACAD-5, ACAD 5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

45.7 kDa (415aa) confirmed by MALDI-TOF

Additionnal Information

GCDH, Glutaryl-CoA dehydrogenase, ACAD5, GCD, Glutaryl-CoA dehydrogenase, ACAD-5, ACAD 5, ATGP0622-10 µg, ATGP0622-20 µg, ATGP0622-50 µg, ATGP0622-100 µg, ATGP0622-250 µg, ATGP0622-500 µg, ATGP0622-1 mg, ATGP0622-10, ATGP0622-20, ATGP0622-50, ATGP0622-100, ATGP0622-250, ATGP0622-500, ATGP0622-1

References & Citations

Keyser B., et al. (2008) Hum. Mol. Genet. 17:3854-3863; ; Westover JB., et al. (2001) Biochemistry. 40 (46) :14106-14.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 200 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_000150

Uniprot Accession Number

Q92947

Species

Human

AA Sequence

MRPEFDWQDP LVLEEQLTTD EILIRDTFRT YCQERLMPRI LLANRNEVFH REIISEMGEL GVLGPTIKGY GCAGVSSVAY GLLARELERV DSGYRSAMSV QSSLVMHPIY AYGSEEQRQK YLPQLAKGEL LGCFGLTEPN SGSDPSSMET RAHYNSSNKS YTLNGTKTWI TNSPMADLFV VWARCEDGCI RGFLLEKGMR GLSAPRIQGK FSLRASATGM IIMDGVEVPE ENVLPGASSL GGPFGCLNNA RYGIAWGVLG ASEFCLHTAR QYALDRMQFG VPLARNQLIQ KKLADMLTEI TLGLHACLQL GRLKDQDKAA PEMVSLLKRN NCGKALDIAR QARDMLGGNG ISDEYHVIRH AMNLEAVNTY EGTHDIHALI LGRAITGIQA FTASK