Recombinant human GCDH protein
Glutaryl-CoA dehydrogenase (GCDH) belongs to the acyl-CoA dehydrogenase family. GCDH catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. This Protein uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. Recombinant GCDH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glutaryl-CoA dehydrogenase, ACAD5, GCD, Glutaryl-CoA dehydrogenase, ACAD-5, ACAD 5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
45.7 kDa (415aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Keyser B., et al. (2008) Hum. Mol. Genet. 17:3854-3863; ; Westover JB., et al. (2001) Biochemistry. 40 (46) :14106-14.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 200 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_000150
Uniprot Accession Number
Q92947
Species
Human
AA Sequence
Frequently Asked Questions
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