Recombinant human GALM protein

GALM (Galactose mutarotase), also known as Aldose 1-epimerase, is a key enzyme of carbohydrate metabolism catalysing the conversion of beta-D-galactose to alpha-D-galactose. This protein may be essential for normal galactose metabolism by maintaining the equilibrium of alpha and beta anomers of galactose. Also it is required for the production of complex oligosaccharides. Recombinant human GALM, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Galctose mutarotase, Aldose 1-epimerase, BLOCK25, IBD1, Galctose mutarotase, Aldose1-epimerase, BLOCK 25, BLOCK-25

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

39.9 kDa (362aa) confirmed by MALDI-TOF

Additionnal Information

GALM, Galctose mutarotase, Aldose 1-epimerase, BLOCK25, IBD1, Galctose mutarotase, Aldose1-epimerase, BLOCK 25, BLOCK-25, ATGP0620-10 µg, ATGP0620-20 µg, ATGP0620-50 µg, ATGP0620-100 µg, ATGP0620-250 µg, ATGP0620-500 µg, ATGP0620-1 mg, ATGP0620-10, ATGP0620-20, ATGP0620-50, ATGP0620-100, ATGP0620-250, ATGP0620-500, ATGP0620-1

References & Citations

Ross AC., et al. (2007) Biochemistry. 46 (51) :15198-207; ; Reece RJ., et al (2003) FEBS Lett. 543 (1-3) :21-4.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_620156

Uniprot Accession Number

Q96C23

Species

Human

AA Sequence

MASVTRAVFG ELPSGGGTVE KFQLQSDLLR VDIISWGCTI TALEVKDRQG RASDVVLGFA ELEGYLQKQP YFGAVIGRVA NRIAKGTFKV DGKEYHLAIN KEPNSLHGGV RGFDKVLWTP RVLSNGVQFS RISPDGEEGY PGELKVWVTY TLDGGELIVN YRAQASQATP VNLTNHSYFN LAGQASPNIN DHEVTIEADT YLPVDETLIP TGEVAPVQGT AFDLRKPVEL GKHLQDFHLN GFDHNFCLKG SKEKHFCARV HHAASGRVLE VYTTQPGVQF YTGNFLDGTL KGKNGAVYPK HSGFCLETQN WPDAVNQPRF PPVLLRPGEE YDHTTWFKFS VA