Recombinant human Lactate Dehydrogenase B/LDHB protein

LDHB, also known as L-lactate dehydrogenase B chain, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD) . The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant human LDHB protein, fused to His-tagging at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 300 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Product Name Alternative

Renal carcinoma antigen NY-REN-46, Lactate dehydrogenase B, L-lactate dehydrogenase B chain, LDH-H, LDH-B, LDH heart subunit

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 300unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Molecular Weight

38.8 kDa (354aa) confirmed by MALDI-TOF

Additionnal Information

Lactate Dehydrogenase B, LDHB, ATGP0616-10 µg, ATGP0616-20 µg, ATGP0616-50 µg, ATGP0616-100 µg, ATGP0616-250 µg, ATGP0616-500 µg, ATGP0616-1 mg, ATGP0616-10, ATGP0616-20, ATGP0616-50, ATGP0616-100, ATGP0616-250, ATGP0616-500, ATGP0616-1

References & Citations

Read JA., et al. (2001) Proteins. 43 (2) :175-85. ; ; Butt AA., et al. (2002) AIDS. 12 (7) :317-21. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_002291.1

Uniprot Accession Number

P07195

Species

Human

AA Sequence

MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL