Recombinant human DCTD protein
DCTD (deoxycytidylate deaminase), also known as dCMP deaminase, is a 178 amino acid allosteric enzyme that exists as a homohexamer and belongs to the cytidine and deoxycytidylate deaminase protein family. using zinc as a cofactor, DCTD catalyzes the deamination of dCMP to duMP, thereby producing the nucleotide substrate (duMP) that is used by thymidylate synthase. Recombinant human DCTD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
Product Specifications
Product Name Alternative
Deoxycytidylate deaminase, dCMP deaminase, MGC111062
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
22.1 kDa (198aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Okazaki T., et al. (2009) Clin Cancer Res. 16 (1) :320-9.; ; Pazy Y., et al. (2009) J Mol Biol. 392 (5) :1205-20.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 20% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001912
Uniprot Accession Number
P32321
Species
Human
AA Sequence
Frequently Asked Questions
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