Recombinant human DCTD protein

DCTD (deoxycytidylate deaminase), also known as dCMP deaminase, is a 178 amino acid allosteric enzyme that exists as a homohexamer and belongs to the cytidine and deoxycytidylate deaminase protein family. using zinc as a cofactor, DCTD catalyzes the deamination of dCMP to duMP, thereby producing the nucleotide substrate (duMP) that is used by thymidylate synthase. Recombinant human DCTD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Deoxycytidylate deaminase, dCMP deaminase, MGC111062

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

22.1 kDa (198aa) confirmed by MALDI-TOF

Additionnal Information

DCTD, Deoxycytidylate deaminase, dCMP deaminase, MGC111062, ATGP0615-10 µg, ATGP0615-20 µg, ATGP0615-50 µg, ATGP0615-100 µg, ATGP0615-250 µg, ATGP0615-500 µg, ATGP0615-1 mg, ATGP0615-10, ATGP0615-20, ATGP0615-50, ATGP0615-100, ATGP0615-250, ATGP0615-500, ATGP0615-1

References & Citations

Okazaki T., et al. (2009) Clin Cancer Res. 16 (1) :320-9.; ; Pazy Y., et al. (2009) J Mol Biol. 392 (5) :1205-20.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 20% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001912

Uniprot Accession Number

P32321

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ