Recombinant human UROD protein

UROD is the fifth enzyme in the human heme biosynthetic pathway and is responsible for the conversion of uroporphyrinogen to coproporphyrinogen through the removal of four carboxymethyl side chains. Mutations and deficiency in this enzyme are responsible for three autosomal disorders in humans: familial porphyria cutanea tarda (f-PCT), sporadic porphyria cutanea tarda (s-PCT) and hepatoerythropoietic porphyria (HEP) . Recombinant human UROD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Uroporphyrinogen decarboxylase, PCT, uroporphyrinogen decarboxylase, uPD, uRO D, uRO-D

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

43.0 kDa (387aa) confirmed by MALDI-TOF

Additionnal Information

UROD, uroporphyrinogen decarboxylase, PCT, uroporphyrinogen decarboxylase, uPD, uRO D, uRO-D, ATGP0612-10 µg, ATGP0612-20 µg, ATGP0612-50 µg, ATGP0612-100 µg, ATGP0612-250 µg, ATGP0612-500 µg, ATGP0612-1 mg, ATGP0612-10, ATGP0612-20, ATGP0612-50, ATGP0612-100, ATGP0612-250, ATGP0612-500, ATGP0612-1

References & Citations

Lewis CA Jr., et al. (2008) Proc Natl Acad Sci u S A. 105 (45) :17328-33. ; ; Garey JR., et al. (1989) Blood. 73 (4) :892-5. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT, 0.1M NaCl, and 1mM EDTA

Scientific Category

Enzymes & Proteases

NCBI Accession Number

AAH01778

Uniprot Accession Number

P06132

Species

Human

AA Sequence

MEANGLGPQG FPELKNDTFL RAAWGEETDY TPVWCMRQAG RYLPEFRETR AAQDFFSTCR SPEACCELTL QPLRRFPLDA AIIFSDILVV PQALGMEVTM VPGKGPSFPE PLREEQDLER LRDPEVVASE LGYVFQAITL TRQRLAGRVP LIGFAGAPWT LMTYMVEGGG SSTMAQAKRW LYQRPQASHQ LLRILTDALV PYLVGQVVAG AQALQLFESH AGHLGPQLFN KFALPYIRDV AKQVKARLRE AGLAPVPMII FAKDGHFALE ELAQAGYEVV GLDWTVAPKK ARECVGKTVT LQVNLDPCAL YASEEEIGQL VKQMLDDFGP HRYIANLGHG LYPDMDPEHV GAFVDAVHKH SRLLRQN