Recombinant human UROD protein
UROD is the fifth enzyme in the human heme biosynthetic pathway and is responsible for the conversion of uroporphyrinogen to coproporphyrinogen through the removal of four carboxymethyl side chains. Mutations and deficiency in this enzyme are responsible for three autosomal disorders in humans: familial porphyria cutanea tarda (f-PCT), sporadic porphyria cutanea tarda (s-PCT) and hepatoerythropoietic porphyria (HEP) . Recombinant human UROD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Uroporphyrinogen decarboxylase, PCT, uroporphyrinogen decarboxylase, uPD, uRO D, uRO-D
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
43.0 kDa (387aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lewis CA Jr., et al. (2008) Proc Natl Acad Sci u S A. 105 (45) :17328-33. ; ; Garey JR., et al. (1989) Blood. 73 (4) :892-5. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT, 0.1M NaCl, and 1mM EDTA
Scientific Category
Enzymes & Proteases
NCBI Accession Number
AAH01778
Uniprot Accession Number
P06132
Species
Human
AA Sequence
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