Recombinant human SUMO E1/SAE1 protein
SAE1, also known as AOS1, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the uBL1 conjugation pathway. Proteins conjugated to ub are marked for progressive degradation by the 26S Proteasome. SAE1, which is a dimeric enzyme, functions as a uBLI E1 ligase mediating the ATP-dependent activation of uBL1. This protein can bind with uBLE1A and uBLE1B to form a heterodimer which can bind uBL1. Recombinant human SAE1 protein, fused to His-T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
SUMO1 activating enzyme subunit 1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, activator Of sumo 1, AOS1, SUA1, UBLE1A
Expression System
E.coli
Antigen Species
Human
Tag
His-T7-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
42.2 kDa (378aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Boggio R., et al. (2007) J Biol Chem. 282 (21) :15376-82.; ; Jones MC., et al. (2006) Proc Natl Acad Sci u S A. 103 (44) :16272-7.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Ubiquitination
NCBI Accession Number
NP_005491.1
Uniprot Accession Number
Q9UBE0
Species
Human
AA Sequence
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