Recombinant human MECR protein

MECR (Mitochondrial trans-2-enoyl-CoA reductase), also known as NRBF1, catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH dependent manner with preference to medium chain length substrate. This protein has a role in the mitochondrial synthesis of fatty acids. Recombinant human MECR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Trans-2-enoyl-CoA reductase mitochondrial, NRBF1, CGI-63, FASN2B, Trans-2-enoyl-CoA reductase, mitochondrial, HsNrbf1, CGI 63, Homolog of yeast 2 enoyl thioester reductase, NBRF 1, NBRF1, NRBF 1, NRBF1, Nuclear receptor binding factor 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.9 kDa (341aa) confirmed by MALDI-TOF

Additionnal Information

MECR, Trans-2-enoyl-CoA reductase mitochondrial, NRBF1, CGI-63, FASN2B, Trans-2-enoyl-CoA reductase, mitochondrial, HsNrbf1, CGI 63, Homolog of yeast 2 enoyl thioester reductase, NBRF 1, NBRF1, NRBF 1, NRBF1, Nuclear receptor binding factor 1, ATGP0607-10 µg, ATGP0607-20 µg, ATGP0607-50 µg, ATGP0607-100 µg, ATGP0607-250 µg, ATGP0607-500 µg, ATGP0607-1 mg, ATGP0607-10, ATGP0607-20, ATGP0607-50, ATGP0607-100, ATGP0607-250, ATGP0607-500, ATGP0607-1

References & Citations

Scicluna EA., et al. (2010) Eur J Clin Microbiol Infect Dis. 29 (2) :163-70.; ; Chen ZJ., et al. (2008) J Mol Biol. 379 (4) :830-44.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

AAH01419

Uniprot Accession Number

Q9BV79

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MPAKVVELKN LELAAVRGSD VRVKMLAAPI NPSDINMIQG NYGLLPELPA VGGNEGVAQV VAVGSNVTGL KPGDWVIPAN AGLGTWRTEA VFSEEALIQV PSDIPLQSAA TLGVNPCTAY RMLMDFEQLQ PGDSVIQNAS NSGVGQAVIQ IAAALGLRTI NVVRDRPDIQ KLSDRLKSLG AEHVITEEEL RRPEMKNFFK DMPQPRLALN CVGGKSSTEL LRQLARGGTM VTYGGMAKQP VVASVSLLIF KDLKLRGFWL SQWKKDHSPD QFKELILTLC DLIRRGQLTA PACSQVPLQD YQSALEASMK PFISSKQILT M