Recombinant human Adenosylhomocysteinease/AHCY protein

AHCY, also known as adenosylhomocysteinase, is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy) . It regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Recombinant human AHCY protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Adenosylhomocysteinase isoform 1, AHCY, AdoHcyase, SAHH, S-adenosylhomocysteine hydrolase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

49.8 kDa (452aa) confirmed by MALDI-TOF

Additionnal Information

Adenosylhomocysteinease, AHCY, ATGP0606-10 µg, ATGP0606-20 µg, ATGP0606-50 µg, ATGP0606-100 µg, ATGP0606-250 µg, ATGP0606-500 µg, ATGP0606-1 mg, ATGP0606-10, ATGP0606-20, ATGP0606-50, ATGP0606-100, ATGP0606-250, ATGP0606-500, ATGP0606-1

References & Citations

Hershfield MS, et al. (1978) Science 202 (4369) :757-60; ; Gellekink H, et al. (2004) Eur J Hum Genet.. 12 (11) :942-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000678.1

Uniprot Accession Number

P23526

Species

Human

AA Sequence

MSDKLPYKVA DIGLAAWGRK ALDIAENEMP GLMRMRERYS ASKPLKGARI AGCLHMTVET AVLIETLVTL GAEVQWSSCN IFSTQDHAAA AIAKAGIPVY AWKGETDEEY LWCIEQTLYF KDGPLNMILD DGGDLTNLIH TKYPQLLPGI RGISEETTTG VHNLYKMMAN GILKVPAINV NDSVTKSKFD NLYGCRESLI DGIKRATDVM IAGKVAVVAG YGDVGKGCAQ ALRGFGARVI ITEIDPINAL QAAMEGYEVT TMDEACQEGN IFVTTTGCID IILGRHFEQM KDDAIVCNIG HFDVEIDVKW LNENAVEKVN IKPQVDRYRL KNGRRIILLA EGRLVNLGCA MGHPSFVMSN SFTNQVMAQI ELWTHPDKYP VGVHFLPKKL DEAVAEAHLG KLNVKLTKLT EKQAQYLGMS CDGPFKPDHY RY