Recombinant human PECI/ECI2 protein

PECI, also referred to as DRS1, ACBD2 or HCA88, is a protein that localizes to the peroxisomal matrix and contains one ACB (acyl-CoA-binding) domain. This protein is expressed abundantly in liver, heart and skeletal muscle. It functions to catalyze the isomerization of both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species, playing an important role in the beta-oxidation of unsaturated fatty acids. Recombinant human PECI protein, fused to his-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Enoyl-CoA delta isomerase 2, D3, D2-enoyl-CoA isomerase, Diazepam-binding inhibitor-related protein 1, DBI-related protein 1, Dodecenoyl-CoA isomerase, Hepatocellular carcinoma-associated antigen 88, Peroxisomal 3,2-trans-enoyl-CoA isomerase, Renal carcinoma antigen NY-REN-1, ACBD2, DRS1, HCA88

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

42.3 kDa (384aa) confirmed by MALDI-TOF

Additionnal Information

PECI, Peroxisomal 32-trans-enoyl-CoA isomerase, ACBD2, DRS1, HCA88, Peroxisomal 3,2-trans-enoyl-CoA isomerase, acyl Coenzyme A binding domain containing 2, D3 D2 enoyl CoA isomerase, DBI related protein 1, Delta (3) delta (2) enoyl CoA isomerase, Dodecenoyl CoA isomerase, DRS 1, HCA 88, Hepatocellular carcinoma associated antigen 88, Peroxisomal D3 D2 enoyl CoA isomerase, Renal carcinoma antigen NY REN 1, ATGP0605-10 µg, ATGP0605-20 µg, ATGP0605-50 µg, ATGP0605-100 µg, ATGP0605-250 µg, ATGP0605-500 µg, ATGP0605-1 mg, ATGP0605-10, ATGP0605-20, ATGP0605-50, ATGP0605-100, ATGP0605-250, ATGP0605-500, ATGP0605-1

References & Citations

Dongyan Zhang, et al. (2002) J. Biol. Chem. 277: 9127-9132.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_006108

Uniprot Accession Number

O75521

Species

Human

AA Sequence

MNRTAMRASQ KDFENSMNQV KLLKKDPGNE VKLKLYALYK QATEGPCNMP KPGVFDLINK AKWDAWNALG SLPKEAARQN YVDLVSSLSP SLESSSQVEP GTDRKSTGFE TLVVTSEDGI TKIMFNRPKK KNAINTEMYH EIMRALKAAS KDDSIITVLT GNGDYYSSGN DLTNFTDIPP GGVEEKAKNN AVLLREFVGC FIDFPKPLIA VVNGPAVGIS VTLLGLFDAV YASDRATFHT PFSHLGQSPE GCSSYTFPKI MSPAKATEML IFGKKLTAGE ACAQGLVTEV FPDSTFQKEV WTRLKAFAKL PPNALRISKE VIRKREREKL HAVNAEECNV LQGRWLSDEC TNAVVNFLSR KSKL