Recombinant human FKBP22/FKBP14 protein

FKBP14, also known as 22 kDa FK506-binding protein, is an enzyme that accelerates the folding of proteins during protein synthesis. This protein contains two EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 greatly reduces peptidyl prolyl cis-trans isomerase activity, therefore suggesting that the PPIase FKBP-type domain must be located at the N-terminus. Recombinant human FKBP14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography

Product Specifications

Specifications

Specific activity is > 240 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.

Product Name Alternative

22 kDa FK506 binding protein, FK506 binding protein 14 (22 kDa), FKBP22, Peptidyl prolyl cis trans isomerase, Peptidyl-prolyl cis-trans isomerase FKBP14, PPIase, Rotamase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 240 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.

Molecular Weight

24.2 kDa (213aa) confirmed by MALDI-TOF

Additionnal Information

22 kDa FK506 binding protein, FK506 binding protein 14, FK506 binding protein 14 (22 kDa), FKBP 22, FKBP22, Peptidyl prolyl cis trans isomerase, Peptidyl-prolyl cis-trans isomerase FKBP14, PPIase, Rotamase, FKBP14, FKBP 14, ATGP0604-10 µg, ATGP0604-20 µg, ATGP0604-50 µg, ATGP0604-100 µg, ATGP0604-250 µg, ATGP0604-500 µg, ATGP0604-1 mg, ATGP0604-010, ATGP0604-020, ATGP0604-050, ATGP0604-100, ATGP0604-250, ATGP0604-500, ATGP0604-01M

References & Citations

Tremmel D., et al. (2007) J Mol Biol. 369 (1) :55-68.; ; Budiman C., et al. (2009) FEBS J. 276 (15) :4091-101.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_060416

Uniprot Accession Number

Q9NWM8

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MALIPEPEVK IEVLQKPFIC HRKTKGGDLM LVHYEGYLEK DGSLFHSTHK HNNGQPIWFT LGILEALKGW DQGLKGMCVG EKRKLIIPPA LGYGKEGKGK IPPESTLIFN IDLLEIRNGP RSHESFQEMD LNDDWKLSKD EVKAYLKKEF EKHGAVVNES HHDALVEDIF DKEDEDKDGF ISAREFTYKH DEL