Recombinant human Transgelin/TAGLN protein

TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein that belongs to the calponin family. This protein is expressed abundantly in fibroblasts and smooth muscle. It is involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. During embryogenesis, TAGLN is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. It is downregulated in several transformed cell lines, indicating that a reduction of TAGLN expression may be an early indicator of the onset of transformation. Recombinant human TAGLN protein, fused to his-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

WS3-10, Transgelin, TAGLN1, Smooth muscle protein 22-alpha, SMCC, SM22 alpha, SM22

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

24.8 kDa (221aa) confirmed by MALDI-TOF

Additionnal Information

SM 22, SM22, SM22 alpha, SM22-alpha, SMCC, Smooth muscle protein 22 alpha, Smooth muscle protein 22-alpha, TAGLN, TAGLN 1, TAGLN1, TAGLN-1, Transgelin, WS3 10, WS310, WS3-10, ATGP0602-10 µg, ATGP0602-20 µg, ATGP0602-50 µg, ATGP0602-100 µg, ATGP0602-250 µg, ATGP0602-500 µg, ATGP0602-1 mg, ATGP0602-010, ATGP0602-020, ATGP0602-050, ATGP0602-100, ATGP0602-250, ATGP0602-500, ATGP0602-01M

References & Citations

Kobayashi R., et al. (1994) Biochem Biophys Res Commun. 198 (3) :1275-80. ; ; Thweatt R., et al. (1992) Biochem Biophys Res Commun. 187 (1) :1-7. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_001001522

Uniprot Accession Number

Q01995

Species

Human

AA Sequence

MANKGPSYGM SREVQSKIEK KYDEELEERL VEWIIVQCGP DVGRPDRGRL GFQVWLKNGV ILSKLVNSLY PDGSKPVKVP ENPPSMVFKQ MEQVAQFLKA AEDYGVIKTD MFQTVDLFEG KDMAAVQRTL MALGSLAVTK NDGHYRGDPN WFMKKAQEHK REFTESQLQE GKHVIGLQMG SNRGASQAGM TGYGRPRQII S