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Recombinant human SLPI protein

SLPI is a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes; the protein is also thought to have broad-spectrum anti-biotic activity. Recombinant human SLPI, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Secretory leukocyte peptidase inhibitor, Antileukoproteinase, ALK1, ALP, BLPI, HuSI, HuSI-I, MPI, WAP4, WFDC4, secretory leukocyte peptidase inhibitor, ALK 1, Antileukoproteinase 1, Antileukoproteinase1, HuSI 1, HuSI I, Protease inhibitor WAP4, Secretory leukocyte protease inhibitor, Seminal proteinase inhibitor, WAP four disulfide core domain protein 4, WAP 4, WFDC 4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

14 kDa (128aa) confirmed by MALDI-TOF

Additionnal Information

SLPI, secretory leukocyte peptidase inhibitor, Antileukoproteinase, ALK1, ALP, BLPI, HuSI, HuSI-I, MPI, WAP4, WFDC4, secretory leukocyte peptidase inhibitor, ALK 1, Antileukoproteinase 1, Antileukoproteinase1, HuSI 1, HuSI I, Protease inhibitor WAP4, Secretory leukocyte protease inhibitor, Seminal proteinase inhibitor, WAP four disulfide core domain protein 4, WAP 4, WFDC 4, ATGP0599-10 µg, ATGP0599-20 µg, ATGP0599-50 µg, ATGP0599-100 µg, ATGP0599-250 µg, ATGP0599-500 µg, ATGP0599-1 mg, ATGP0599-10, ATGP0599-20, ATGP0599-50, ATGP0599-100, ATGP0599-250, ATGP0599-500, ATGP0599-1

References & Citations

Rasool N., et al. (2010) Clin Cancer Res. 16 (2) :600-9; ; King AE., et al. (2009) Hum Reprod. 24 (3) :679-86.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100 mM NaCl

Scientific Category

Immunology

NCBI Accession Number

NP_003055.1

Uniprot Accession Number

P03973

Species

Human

AA Sequence

SGKSFKAGV CPPKKSAQCL RYKKPECQSD WQCPGKKRCC PDTCGIKCLD PVDTPNPTRR KPGKCPVTYG QCLMLNPPNF CEMDGQCKRD LKCCMGMCGK SCVSPVKA

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