Recombinant human Rap1A protein
Ras-related protein Rap-1A (RAP1A) belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. This Protein Induces morphological reversion of a cell line transformed by a Ras oncogene and Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Recombinant human RAP1A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
22.8 kDa (201aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Han, L., et al. (1995) Mol Cell Biol. 15 (3) : 1318-23.; ; Rebhun, J F, et al. (2000) J Biol Chem. 275 (45) : 34901-8.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 5mM DTT, 200 mM NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001010935
Uniprot Accession Number
P62834
Species
Human
AA Sequence
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