Recombinant human CHP protein

CHP (Calcium-binding protein P22) is a phosphoprotein that binds to the sodium-hydrogen exchangers (NHEs) . This protein serves as an essential cofactor which supports the physiological activity of NHE family members. It has protein sequence similarity to calcineurin B and it is also known to be an endogenous inhibitor of calcineurin activity. Recombinant human CHP, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Calcium-binding protein p22, SLC9A1BP, Calcineurin B homolog, Calcineurin homologous protein, Calcium-binding protein p22, Calcium binding protein CHP, Sid 470, Sid470, SLC9A1 binding protein, Wrch2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

24.7 kDa (216aa) confirmed by MALDI-TOF

Additionnal Information

CHP, Calcium-binding protein p22, SLC9A1BP, Calcineurin B homolog, Calcineurin homologous protein, Calcium-binding protein p22, Calcium binding protein CHP, Sid 470, Sid470, SLC9A1 binding protein, Wrch2, ATGP0597-10 µg, ATGP0597-20 µg, ATGP0597-50 µg, ATGP0597-100 µg, ATGP0597-250 µg, ATGP0597-500 µg, ATGP0597-1 mg, ATGP0597-10, ATGP0597-20, ATGP0597-50, ATGP0597-100, ATGP0597-250, ATGP0597-500, ATGP0597-1

References & Citations

Shigekawa M., et al. (2001) J Biol Chem. 276 (20) :17367-72.; ; Kojima C., et al (2007) J Biol Chem. 282 (4) :2741-51.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_009167.1

Uniprot Accession Number

Q99653

Species

Human

AA Sequence

MGSRASTLL RDEELEEIKK ETGFSHSQIT RLYSRFTSLD KGENGTLSRE DFQRIPELAI NPLGDRIINA FFPEGEDQVN FRGFMRTLAH FRPIEDNEKS KDVNGPEPLN SRSNKLHFAF RLYDLDKDEK ISRDELLQVL RMMVGVNISD EQLGSIADRT IQEADQDGDS AISFTEFVKV LEKVDVEQKM SIRFLH