Recombinant human eIF-5A2/EIF5A2 protein
EIF5A2, a member of eukaryotic initiation factor 5A subfamily, is an essential protein tightly linked to cellular polyamine homeostasis. The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond during the initial stage of protein synthesis. It seems to be the only eukaryotic protein to have a hypusine residue, which is a post-translational modification of a lysine by the addition of a butylamino group. Recombinant human EIF5A2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
MGC124093, MGC124092, Eukaryotic translation initiation factor 5A2, Eukaryotic initiation factor 5A isoform 2, eIF5AII, EIF5A2, eIF-4D
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
18.9 kDa (173aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Guan XY., et al. (2010) Hepatology. 51 (4) :1255-63.; ; Luk JM., et al. (2009) Int J Cancer. 127 (4) :968-76.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_065123
Uniprot Accession Number
Q9GZV4
Species
Human
AA Sequence
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