Recombinant human eIF-5A2/EIF5A2 protein

EIF5A2, a member of eukaryotic initiation factor 5A subfamily, is an essential protein tightly linked to cellular polyamine homeostasis. The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond during the initial stage of protein synthesis. It seems to be the only eukaryotic protein to have a hypusine residue, which is a post-translational modification of a lysine by the addition of a butylamino group. Recombinant human EIF5A2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

MGC124093, MGC124092, Eukaryotic translation initiation factor 5A2, Eukaryotic initiation factor 5A isoform 2, eIF5AII, EIF5A2, eIF-4D

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

18.9 kDa (173aa) confirmed by MALDI-TOF

Additionnal Information

MGC124093, MGC124092, Eukaryotic translation initiation factor 5A2, Eukaryotic initiation factor 5A isoform 2, eIF5AII, EIF5A2, EIF5A 2, eIF-4D, eIF 5A2, eIF 5A 2, ATGP0596-10 µg, ATGP0596-20 µg, ATGP0596-50 µg, ATGP0596-100 µg, ATGP0596-250 µg, ATGP0596-500 µg, ATGP0596-1 mg, ATGP0596-010, ATGP0596-020, ATGP0596-050, ATGP0596-100, ATGP0596-250, ATGP0596-500, ATGP0596-01M

References & Citations

Guan XY., et al. (2010) Hepatology. 51 (4) :1255-63.; ; Luk JM., et al. (2009) Int J Cancer. 127 (4) :968-76.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_065123

Uniprot Accession Number

Q9GZV4

Species

Human

AA Sequence

MADEIDFTTG DAGASSTYPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDYQ LICIQDGYLS LLTETGEVRE DLKLPEGELG KEIEGKYNAG EDVQVSVMCA MSEEYAVAIK PCK