Recombinant human NTF2/NUTF2 protein
NuTF2 is a cytosolic factor that facilitates protein transport into the nucleus. It interacts with the nuclear pore complex glycoprotein p62 and Ran. This protein acts at a relative late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. It binds directly to RanGDP and may modulate the steady-state distribution of Ran. Recombinant human NuTF2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Nuclear transport factor 2, NTF2, Placental protein 15, PP15, NuTF 2, PP 15
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
16.6 kDa (147aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gerace L., et al. (1995) J Cell Biol. 129 (4) :925-37.; ; Tang SB., et al. (2009) Mol Vis. 15:861-9.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Adhesion & Movement
NCBI Accession Number
NP_005787
Uniprot Accession Number
P61970
Species
Human
AA Sequence
MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH QMFLLKNIND AWVCTNDMFR LALHNFG
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