Recombinant human MAGEA4 protein

MAGEA4 is a member of the MAGEA family, which directs the expression of tumor antigens that are recognized on a human melanoma by autologous cytolytic T lymphocytes (CTL) . The function of MAGEA4 is not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression. This protein is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Recombinant human MAGEA4, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Melanoma-associated antigen 4, Melanoma-associated antigen 4, Cancer/testis antigen 1.4, CT1.4, MAGE 4, MAGE 4 antigen, MAGE 41, MAGE 41 antigen, MAGE 4A, MAGE 4B, MAGE X2, MAGE X2 antigen, MAGE41, MAGE4A, MAGE4B, MAGEA 4, MAGEA4, Melanoma antigen family A 4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

37 kDa (337aa) confirmed by MALDI-TOF

Additionnal Information

MAGEA4, ATGP0594-10 µg, ATGP0594-20 µg, ATGP0594-50 µg, ATGP0594-100 µg, ATGP0594-250 µg, ATGP0594-500 µg, ATGP0594-1 mg, ATGP0594-10, ATGP0594-20, ATGP0594-50, ATGP0594-100, ATGP0594-250, ATGP0594-500, ATGP0594-1

References & Citations

Fradet Y., et al. (2009) Int J Cancer. 125 (6) :1365-71.; ; Nishimura T., et al. (2009) Br J Cancer. 100 (7) :1135-43.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Scientific Category

Cancer

NCBI Accession Number

AAH17723.1

Uniprot Accession Number

P43358

Species

Human

AA Sequence

MSSEQKSQHC KPEEGVEAQE EALGLVGAQA PTTEEQEAAV SSSSPLVPGT LEEVPAAESA GPPQSPQGAS ALPTTISFTC WRQPNEGSSS QEEEGPSTSP DAESLFREAL SNKVDELAHF LLRKYRAKEL VTKAEMLERV IKNYKRCFPV IFGKASESLK MIFGIDVKEV DPTSNTYTLV TCLGLSYDGL LGNNQIFPKT GLLIIVLGTI AMEGDSASEE EIWEELGVMG VYDGREHTVY GEPRKLLTQD WVQENYLEYR QVPGSNPARY EFLWGPRALA ETSYVKVLEH VVRVNARVRI AYPSLREAAL LEEEEGV