Recombinant human MAGEA4 protein
MAGEA4 is a member of the MAGEA family, which directs the expression of tumor antigens that are recognized on a human melanoma by autologous cytolytic T lymphocytes (CTL) . The function of MAGEA4 is not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression. This protein is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Recombinant human MAGEA4, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
37 kDa (337aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Fradet Y., et al. (2009) Int J Cancer. 125 (6) :1365-71.; ; Nishimura T., et al. (2009) Br J Cancer. 100 (7) :1135-43.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
Scientific Category
Cancer
NCBI Accession Number
AAH17723.1
Uniprot Accession Number
P43358
Species
Human
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Total Carbohydrate Colorimetric Microplate Assay Kit
