Recombinant E.coli skp protein

The Seventeen Kilodalton Protein (Skp) is a trimeric periplasmic chaperone that assists outer membrane proteins in their folding and insertion into membranes. This protein required for the efficient release of ompA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of ompA into the outer membrane. Recombinant E. coli Skp, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Chaperone protein skp, Periplasmic chaperone, ompH, DNA binding 17 kDa protein, ECK0177, Histone like protein HLP 1, hlpA, JW0173, Seventeen kilodalton protein

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.9 kDa (162aa) confirmed by MALDI-TOF

Additionnal Information

Skp, Chaperone protein skp, Chaperone protein skp, periplasmic chaperone, ompH, DNA binding 17 kDa protein, ECK0177, Histone like protein HLP 1, hlpA, JW0173, Seventeen kilodalton protein, ATGP0589-10 µg, ATGP0589-20 µg, ATGP0589-50 µg, ATGP0589-100 µg, ATGP0589-250 µg, ATGP0589-500 µg, ATGP0589-1 mg, ATGP0589-10, ATGP0589-20, ATGP0589-50, ATGP0589-100, ATGP0589-250, ATGP0589-500, ATGP0589-1

References & Citations

Korndorfer IP., et al. (2004) Nat Struct Mol Biol. 11 (10) :1015-20; ; Walton TA., et al. (2004) Mol Cell. 15 (3) :367-74.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_414720

Uniprot Accession Number

P0AEU7

Species

E.coli

AA Sequence

ADKIAIVNM GSLFQQVAQK TGVSNTLENE FKGRASELQR METDLQAKMK KLQSMKAGSD RTKLEKDVMA QRQTFAQKAQ AFEQDRARRS NEERGKLVTR IQTAVKSVAN SQDIDLVVDA NAVAYNSSDV KDITADVLKQ VK