Recombinant E.coli skp protein
The Seventeen Kilodalton Protein (Skp) is a trimeric periplasmic chaperone that assists outer membrane proteins in their folding and insertion into membranes. This protein required for the efficient release of ompA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of ompA into the outer membrane. Recombinant E. coli Skp, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
17.9 kDa (162aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Korndorfer IP., et al. (2004) Nat Struct Mol Biol. 11 (10) :1015-20; ; Walton TA., et al. (2004) Mol Cell. 15 (3) :367-74.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_414720
Uniprot Accession Number
P0AEU7
Species
E.coli
AA Sequence
Frequently Asked Questions
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