Recombinant human NCK1 protein

NCK adaptor protein 1, also known as NCK, belongs to the adaptor family of proteins. The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast. Recombinant human NCK1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

NCK adaptor protein 1, NCK, NCKalpha, NCK adaptor protein 1 GRB4, melanoma NCK protein, MGC12668, NCK adaptor protein 2, NCK tyrosine kinase, NCKbeta, non catalytic region of tyrosine kinase, noncatalytic region of tyrosine kinase, beta, SH2/SH3 adaptor protein NCK alpha, SH2/SH3 adaptor protein NCK beta.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

45 kDa (397aa) confirmed by MALDI-TOF

Additionnal Information

NCK1, NCK adaptor protein 1, NCK, NCKalpha, NCK adaptor protein 1 GRB4, melanoma NCK protein, MGC12668, NCK adaptor protein 2, NCK tyrosine kinase, NCKbeta, non catalytic region of tyrosine kinase, noncatalytic region of tyrosine kinase, beta, SH2/SH3 adaptor protein NCK alpha, SH2/SH3 adaptor protein NCK beta., ATGP0587-10 µg, ATGP0587-20 µg, ATGP0587-50 µg, ATGP0587-100 µg, ATGP0587-250 µg, ATGP0587-500 µg, ATGP0587-1 mg, ATGP0587-10, ATGP0587-20, ATGP0587-50, ATGP0587-100, ATGP0587-250, ATGP0587-500, ATGP0587-1

References & Citations

Chen., et al. (1998) J bio Chem. 273 (39) :25171-8.; ; Li W., et al. (1992) Mol Cell Biol. 12 (12) :5824-33.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 1mM EDTA, 50 mM NaCl

Scientific Category

Cancer

NCBI Accession Number

NP_006144

Uniprot Accession Number

P16333

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVIEKPENDPEWW KCRKINGMVG LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME ELVEHYKKAP IFTSEQGEKL YLVKHLS