Recombinant human IMP Dehydrogenase 1/IMPDH1 protein

Inosine-5'-monophosphate dehydrogenase 1, also known as IMPDH1 catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP) . Also, IMPDH1 is a ubiquitously expressed enzyme, functioning as a homotetramer, which catalyzed the rate-limiting step in de novo synthesis of guanine nucleotides. As such, it plays an important role in cyclic nucleoside metabolism within photoreceptors. Defects in the IMPDH1 gene are a cause of retinitis pigmentosa type 10. Recombinant human IMPDH1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Inosine-5'-monophosphate dehydrogenase 1, IMPD, IMPD1, LCA11, RP10, Inosine-5'-monophosphate dehydrogenase 1 IMP dehydrogenase 1.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

57.5 kDa (534aa) confirmed by MALDI-TOF

Additionnal Information

IMP Dehydrogenase1, IMPDH1, Inosine-5'-monophosphate dehydrogenase 1, IMPD, IMPD1, LCA11, RP10, Inosine-5'-monophosphate dehydrogenase 1 IMP dehydrogenase 1., ATGP0586-10 µg, ATGP0586-20 µg, ATGP0586-50 µg, ATGP0586-100 µg, ATGP0586-250 µg, ATGP0586-500 µg, ATGP0586-1 mg, ATGP0586-10, ATGP0586-20, ATGP0586-50, ATGP0586-100, ATGP0586-250, ATGP0586-500, ATGP0586-1

References & Citations

Wang J., et al. (2008) Clin Pharmacol Ther. 83 (5) :711-7.; ; Natsumeda Y., et al. (1990) J Biol Chem. 265 (9) :5292-5.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001136045

Uniprot Accession Number

P20839

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MADYLISGGT GYVPEDGLTA QQLFASADGL TYNDFLILPG FIDFIADEVD LTSALTRKIT LKTPLISSPM DTVTEADMAI AMALMGGIGF IHHNCTPEFQ ANEVRKVKKF EQGFITDPVV LSPSHTVGDV LEAKMRHGFS GIPITETGTM GSKLVGIVTS RDIDFLAEKD HTTLLSEVMT PRIELVVAPA GVTLKEANEI LQRSKKGKLP IVNDCDELVA IIARTDLKKN RDYPLASKDS QKQLLCGAAV GTREDDKYRL DLLTQAGVDV IVLDSSQGNS VYQIAMVHYI KQKYPHLQVI GGNVVTAAQA KNLIDAGVDG LRVGMGCGSI CITQEVMACG RPQGTAVYKV AEYARRFGVP IIADGGIQTV GHVVKALALG ASTVMMGSLL AATTEAPGEY FFSDGVRLKK YRGMGSLDAM EKSSSSQKRY FSEGDKVKIA QGVSGSIQDK GSIQKFVPYL IAGIQHGCQD IGARSLSVLR SMMYSGELKF EKRTMSAQIE GGVHGLHSYE KRLY