Recombinant human IMP Dehydrogenase 1/IMPDH1 protein
Inosine-5'-monophosphate dehydrogenase 1, also known as IMPDH1 catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP) . Also, IMPDH1 is a ubiquitously expressed enzyme, functioning as a homotetramer, which catalyzed the rate-limiting step in de novo synthesis of guanine nucleotides. As such, it plays an important role in cyclic nucleoside metabolism within photoreceptors. Defects in the IMPDH1 gene are a cause of retinitis pigmentosa type 10. Recombinant human IMPDH1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Inosine-5'-monophosphate dehydrogenase 1, IMPD, IMPD1, LCA11, RP10, Inosine-5'-monophosphate dehydrogenase 1 IMP dehydrogenase 1.
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
57.5 kDa (534aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wang J., et al. (2008) Clin Pharmacol Ther. 83 (5) :711-7.; ; Natsumeda Y., et al. (1990) J Biol Chem. 265 (9) :5292-5.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001136045
Uniprot Accession Number
P20839
Species
Human
AA Sequence
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