Recombinant human RRM2 protein

RRM2, also known as ribonucleotide reductase M2, is an enzyme that catalyzes the formation of deoxyribonucleotides from ribonucleotides. This protein plays a critical role in regulating the total rate of DNA synthesis so that DNA to cell mass is maintained at a constant ratio during cell division and DNA repair. Recombinant human RRM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Ribonucleoside-diphosphate reductase subunit M2, R2, RR2, RR2M, Ribonucleoside-diphosphate reductase subunit M2 Ribonucleotide reductase M2 subunit, Ribonucleotide reductase small subunit

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

47 kDa (409aa) confirmed by MALDI-TOF

Additionnal Information

RRM2, Ribonucleoside-diphosphate reductase subunit M2, R2, RR2, RR2M, Ribonucleoside-diphosphate reductase subunit M2 Ribonucleotide reductase M2 subunit, Ribonucleotide reductase small subunit, ATGP0582-10 µg, ATGP0582-20 µg, ATGP0582-50 µg, ATGP0582-100 µg, ATGP0582-250 µg, ATGP0582-500 µg, ATGP0582-1 mg, ATGP0582-10, ATGP0582-20, ATGP0582-50, ATGP0582-100, ATGP0582-250, ATGP0582-500, ATGP0582-1

References & Citations

Torrents E, et al. (2002) J Mol Evol. 55 (2) :138-52; ; Herrick J, et al. (2007) Mol Microbiol. 63 (1) :22-34;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001025.1

Uniprot Accession Number

P31350

Species

Human

AA Sequence

MLSLRVPLAP ITDPQQLQLS PLKGLSLVDK ENTPPALSGT RVLASKTARR IFQEPTEPKT KAAAPGVEDE PLLRENPRRF VIFPIEYHDI WQMYKKAEAS FWTAEEVDLS KDIQHWESLK PEERYFISHV LAFFAASDGI VNENLVERFS QEVQITEARC FYGFQIAMEN IHSEMYSLLI DTYIKDPKER EFLFNAIETM PCVKKKADWA LRWIGDKEAT YGERVVAFAA VEGIFFSGSF ASIFWLKKRG LMPGLTFSNE LISRDEGLHC DFACLMFKHL VHKPSEERVR EIIINAVRIE QEFLTEALPV KLIGMNCTLM KQYIEFVADR LMLELGFSKV FRVENPFDFM ENISLEGKTN FFEKRVGEYQ RMGVMSSPTE NSFTLDADF