Recombinant human CCL19/MIP-3 beta protein
CCL19 is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC) . This protein is involved in immunoregulatory and inflammatory processes. It elicits its effects on its target cells by binding to the chemokine receptor chemokine receptor CCR7. It attracts certain cells of the immune system, including dendritic cells and antigen-engaged B cells, CCR7+ effector-memory T-Cells. Recombinant human CCL19 protein, fused to T7-tag at N-terminus, was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
T7-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
10.4 kDa (93aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Yoshida R, et al. (1997) J Biol Chem. 272 (21) :13803-9.; ; Robbiani DF, et al. (2000) Cell. 103 (5) :757-68.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_006265.1
Uniprot Accession Number
Q99731
Species
Human
AA Sequence
GTND AEDCCLSVTQ KPIPGYIVRN FHYLLIKDGC RVPAVVFTTL RGRQLCAPPD QPWVERIIQR LQRTSAKMKR RSS
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