Recombinant human CERT1 protein

CERT is a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Recombinant human CERT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ceramide transporter 1, Collagen type IV alpha-3-binding protein, Goodpasture antigen-binding protein, GPBP, START domain-containing protein 11, StARD11, StAR-related lipid transfer protein 11, CERT, COL4A3BP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

32.9 kDa (290aa) confirmed by MALDI-TOF

Additionnal Information

CERT, ATGP0579-10 µg, ATGP0579-20 µg, ATGP0579-50 µg, ATGP0579-100 µg, ATGP0579-250 µg, ATGP0579-500 µg, ATGP0579-1 mg, ATGP0579-10, ATGP0579-20, ATGP0579-50, ATGP0579-100, ATGP0579-250, ATGP0579-500, ATGP0579-1

References & Citations

Turner N., et al. (1992) J Clin Invest 89 (2) :592-601.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Scientific Category

Immunology

NCBI Accession Number

NP_112729

Uniprot Accession Number

Q9Y5P4

Species

Human

AA Sequence

LH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL ADNAIIIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VRAKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAVAKREYPK FLKRFTSYVQ EKTAGKPILF