Recombinant human SORD protein
SORD, also known as L-iditol 2-dehydrogenase or SORD1, is a 357 amino acid member of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney and in the lens of the eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses. Recombinant human SORD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze D-fructose to D-sorbitol per minute at pH 7.5 at 37C.
Product Name Alternative
Sorbitol dehydrogenase, Sorbitol dehydrogenase L iditol 2 dehydrogenase, SDH, SORD, SORD 1, SORD1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
Enzyme Activity, SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 20,000pmol/min/ug, and is defined as the amount of enzyme that catalyze D-fructose to D-sorbitol per minute at pH 7.5 at 37C.
Molecular Weight
40.4 kDa (377aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Baker MA., et al. (2010) Proteomics. 10 (3) :482-95. ; ; Sorger D., et al. (2009) Nucl Med Biol. 36 (1) :17-27.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
Scientific Category
Metabolism
NCBI Accession Number
AAH25295
Uniprot Accession Number
Q00796
Species
Human
AA Sequence
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