Recombinant human FHIT protein

FHIT (fragile histidine triad) is an enzyme that cleaves adenosine 5' PPP 5' A to yield AMP and ADP. FHIT gene encompasses the common fragile site FRA3B on chromosome 3. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. This protein physically associates with ubiquitin conjugating enzyme 9. Recombinant human FHIT, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Bis (5'-adenosyl) -triphosphatase, AP3A hydrolase, AP3Aase, Adenosine 5'-monophosphoramidase FHIT, Adenylylsulfatase, Adenylylsulfate-ammonia adenylyltransferase, Diadenosine 5',5'''-P1, P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein, FRA3B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.9 kDa (155aa) confirmed by MALDI-TOF

Additionnal Information

FHIT, Fragile histidine triad protein, Bis (5'-adenosyl) -triphosphatase, AP3Aase, Fragile histidine triad protein AP3A hydrolase, AP3A hydrolase fragile site 3p14.2, Bis 5' adenosyl triphosphatase, Diadenosine 5' 5''' P1 P3 triphosphate hydrolase, Dinucleosidetriphosphatase, FRA 3B, FRA3B, Fragile histidine triad, Fragile histidine triad gene, Fragile histidine triad protein, Tumor suppressor protein., ATGP0563-10 µg, ATGP0563-20 µg, ATGP0563-50 µg, ATGP0563-100 µg, ATGP0563-250 µg, ATGP0563-500 µg, ATGP0563-1 mg, ATGP0563-10, ATGP0563-20, ATGP0563-50, ATGP0563-100, ATGP0563-250, ATGP0563-500, ATGP0563-1

References & Citations

Kmiec Z., et al. (2009) J Physiol Pharmacol. 60 (4) :63-70.; ; Ahmad F., et al. (2010) J Cancer Res Clin Oncol. 136 (3) :333-50.;

Other References

Kawaguchi M, et al. Synthesis of Fluorescent Probes Targeting Tumor-Suppressor Protein FHIT and Identification of Apoptosis-Inducing FHIT Inhibitors. (J Med Chem. 2021) {https://pubmed.ncbi.nlm.nih.gov/34160227/}