Recombinant human FKBP36/FKBP6 protein

FK506 binding protein 6, also known as FKBP6, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. ubiquitously expressed in all tissues, FKBP6 is present at highest levels in testis, liver, kidney, skeletal muscle and heart. Deletion of FKBP6 may contribute to hypercalcemia and growth delay in Williams-Beuren syndrome. Recombinant human FKBP6, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 190 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.

Product Name Alternative

Inactive peptidyl-prolyl cis-trans isomerase FKBP6, Inactive PPIase FKBP6, 36 kDa FK506-binding protein, 36 kDa FKBP, FKBP-36, FK506-binding protein 6, FKBP-6, Immunophilin FKBP36, peptidylprolyl cis-trans isomerase, Rotamase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 190 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.

Molecular Weight

39.3 kDa (347aa) confirmed by MALDI-TOF

Additionnal Information

FKBP6, FK506 binding protein 6 36kDa, FKBP36, PPIase FKBP6, FK506 binding protein 6, 36kDa 36 kDa FK506 binding protein, EC 5.2.1.8, FK506 binding protein 6, FKBP 36, Immunophilin FKBP36, Peptidyl prolyl cis trans isomerase, PPIase, Rotamase., ATGP0560-10 µg, ATGP0560-20 µg, ATGP0560-50 µg, ATGP0560-100 µg, ATGP0560-250 µg, ATGP0560-500 µg, ATGP0560-1 mg, ATGP0560-10, ATGP0560-20, ATGP0560-50, ATGP0560-100, ATGP0560-250, ATGP0560-500, ATGP0560-1

References & Citations

Crackower MA., et al. (2003) Science. 300 (5623) :1231-5.; ; Metcalfe K., et al. (2005) Clin Dysmorphpl. 14 (2) : 61-5.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 1mM EDTA, 0.2M NaCl

Scientific Category

Immunology

NCBI Accession Number

NP_003593.3

Uniprot Accession Number

O75344

Species

Human

AA Sequence

MGGSALNQGV LEGDDAPGQS LYERLSQRML DISGDRGVLK DVIREGAGDL VAPDASVLVK YSGYLEHMDR PFDSNYFRKT PRLMKLGEDI TLWGMELGLL SMRRGELARF LFKPNYAYGT LGCPPLIPPN TTVLFEIELL DFLDCAESDK FCALSAEQQD QFPLQKVLKV AATEREFGNY LFRQNRFYDA KVRYKRALLL LRRRSAPPEE QHLVEAAKLP VLLNLSFTYL KLDRPTIALC YGEQALIIDQ KNAKALFRCG QACLLLTEYQ KARDFLVRAQ KEQPFNHDIN NELKKLASCY RDYVDKEKEM WHRMFAPCGD GSTAGES