Recombinant human Crystallin mu/CRYM protein
Crystallin mu, also known as CRYM, is a member of the crystallin protein family. Crystallins are separated into two classes, taxon-specific and ubiquitous. This gene encodes a taxon-specific crystallin protein. The human CRYM gene maps to chromosome 16p13. 11, and encodes a protein that is expressed in neural tissue, muscle, and kidney. unlike other crystallins, CRYM does not perform a structural role in lens tissue, but rather it binds NADPH and thyroid hormone, which indicates that it may have other regulatory or developmental functions. Recombinant human CRYM, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
35.9 kDa (334aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kim RY., et al. (1992) . Proc Natl Acad Sci uSA. 89 (19) :9292-6.; ; Muaders NW., et al. (1988) J Biol Chem. 263:15462-15466.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_001879
Uniprot Accession Number
Q14894
Species
Human
AA Sequence
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