Recombinant human Adenosine Kinase/ADK protein
Adenosine kinase, also known as ADK is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant human Adenosine kinase, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
ADK, AK, Adenosine kinase, Adenosine 5'-phosphotransferase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
40.5 kDa (362aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Chakraborty, A., et al. (2002) J. Biol. Chem. 277:47451-47451.; ; Sakowicz M., et al. (2001) Acta Biochim Pol. 48 (3) : 745-54.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 1mM EDTA, 50 mM NaCl
Scientific Category
Cancer
NCBI Accession Number
NP_006712
Uniprot Accession Number
P55263
Species
Human
AA Sequence
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