Recombinant human Ubc2/UBE2A protein
Ubiquitin-conjugating enzyme E2 A isoform 1, also known as uBE2A is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. Recombinant human uBE2A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ubiquitin-conjugating enzyme E2 A, RAD6 homolog A, RAD6A, HR6A, hHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A, UBC2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
19.4 kDa (172aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Sarcevic B., et al. (2002) EMBO J. 21 (8) :2009-18.; ; Ciechanover A., et al. (1994) FASEB. 8:182-191.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 1mM EDTA
Scientific Category
Ubiquitination
NCBI Accession Number
NP_003327
Uniprot Accession Number
P49459
Species
Human
AA Sequence
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