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Recombinant human PDE6D protein

Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (alphabeta) that is regulated by two inhibitory gamma subunits. As PDE6D does not modify the catalytic properties of PDEalphabeta, its function is still unresolved. Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Retinal rod rhodopsin-sensitive cGMP 3'5'-cyclic phosphodiesterase subunit delta, PDED, Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta, phosphodiesterase 6D, cGMP-specific, rod, delta

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

18.4 kDa (158aa) confirmed by MALDI-TOF

Additionnal Information

PDE6D, Retinal rod rhodopsin-sensitive cGMP 3'5'-cyclic phosphodiesterase subunit delta, PDED, Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta, phosphodiesterase 6D, cGMP-specific, rod, delta, ATGP0553-10 µg, ATGP0553-20 µg, ATGP0553-50 µg, ATGP0553-100 µg, ATGP0553-250 µg, ATGP0553-500 µg, ATGP0553-1 mg, ATGP0553-10, ATGP0553-20, ATGP0553-50, ATGP0553-100, ATGP0553-250, ATGP0553-500, ATGP0553-1

References & Citations

Zhang H., et al, (2007) Proc Natl Acad Sci u S A. 104 (21) :8857-62.; ; Dekomien., G. et al. (2003) Genet Sel Evol. 35 (4) :445-56.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002592

Uniprot Accession Number

O43924

Species

Human

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