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Recombinant human MED4 protein

Mediator complex subunit 4 (MED4) is also known as mediator of RNA polymerase II transcription subunit 4 or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36) . This protein is a component of the vitamin D receptor-interacting protein (DRIP) complex which functions as a nuclear receptor coactivator. The DRIP complex is capable of activating nuclear receptors in a ligand-dependent manner. Recombinant human MED4, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mediator complex subunit 4, ARC36, DRIP36, VDRIP, Mediator complex subunit 4 Activator-recruited cofactor 36 kDa component, HSPC126, Mediator of RNA polymerase II transcription subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, Vitamin D3 receptor-interacting protein complex 36 kDa component.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

30.7 kDa (278aa) confirmed by MALDI-TOF

Additionnal Information

MED4, Mediator complex subunit 4, ARC36, DRIP36, VDRIP, Mediator complex subunit 4 Activator-recruited cofactor 36 kDa component, HSPC126, Mediator of RNA polymerase II transcription subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, Vitamin D3 receptor-interacting protein complex 36 kDa component., ATGP0552-10 µg, ATGP0552-20 µg, ATGP0552-50 µg, ATGP0552-100 µg, ATGP0552-250 µg, ATGP0552-500 µg, ATGP0552-1 mg, ATGP0552-10, ATGP0552-20, ATGP0552-50, ATGP0552-100, ATGP0552-250, ATGP0552-500, ATGP0552-1

References & Citations

Rachez C., et al. (1999) Nature 398 (6730) : 824-8.; ; Panierakis C., et al. (2009) Int J Infect Dis.13 (6) :e437-43.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 100 mM NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

AAH05189

Uniprot Accession Number

Q9NPJ6

Species

Human

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