Recombinant human Glutaredoxin 3/GLRX3 protein

Glutaredoxin (GRX), also known as thioltransferase, is member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Glutaredoxin-3 (Grx3/PICOT) is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals. And it has been shown to interact with PRKCQ. Recombinant human Glutaredoxin-3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glutaredoxin-3, GRX3, PKC-interacting cousin of thioredoxin, PKC-theta-interacting protein, PKCq-interacting protein, Thioredoxin-like protein 2, Glutaredoxin 4, GLRX4, GRX4, PICOT, TXNL2, HUSSY-22, bA500G10.4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

39.6 kDa (355aa) confirmed by MALDI-TOF

Additionnal Information

TXNL2, TXNL 2, Thioredoxin-like2, Thioredoxin-like protein 2, Thioredoxin-like 2, Thioredoxin like 2, PKC-theta-interacting protein, PKCq-interacting protein, PKCq interacting protein, PKC-interacting cousin of thioredoxin, PKC theta interacting protein, PKC interacting cousin of thioredoxin, PICOT, HUSSY-22, GRX4, GRX3, Glutaredoxin-4, Glutaredoxin4, Glutaredoxin-3, Glutaredoxin3, Glutaredoxin 4, Glutaredoxin 3, GLRX4, GLRX3, bA500G10.4, ATGP0551-10 µg, ATGP0551-20 µg, ATGP0551-50 µg, ATGP0551-100 µg, ATGP0551-250 µg, ATGP0551-500 µg, ATGP0551-1 mg, ATGP0551-010, ATGP0551-020, ATGP0551-050, ATGP0551-100, ATGP0551-250, ATGP0551-500, ATGP0551-01M

References & Citations

Witte S., et al. (2000) J Biol Chem 275 (3) : 1902-9.; ; Haunhorst P., et al. (2010) Biochem Biophys Res Commun. 394 (2) :372-6;

Other References

Kamisuki S, et al. Identification of proteins that bind to the neuroprotective agent neoechinulin A. (Biosci Biotechnol Biochem. 2018) {https://pubmed.ncbi.nlm.nih.gov/29447077/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Redox Proteins

NCBI Accession Number

NP_006532

Uniprot Accession Number

O76003

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAAGAAEAAV AAVEEVGSAG QFEELLRLKA KSLLVVHFWA PWAPQCAQMN EVMAELAKEL PQVSFVKLEA EGVPEVSEKY EISSVPTFLF FKNSQKIDRL DGAHAPELTK KVQRHASSGS FLPSANEHLK EDLNLRLKKL THAAPCMLFM KGTPQEPRCG FSKQMVEILH KHNIQFSSFD IFSDEEVRQG LKAYSSWPTY PQLYVSGELI GGLDIIKELE ASEELDTICP KAPKLEERLK VLTNKASVML FMKGNKQEAK CGFSKQILEI LNSTGVEYET FDILEDEEVR QGLKAYSNWP TYPQLYVKGE LVGGLDIVKE LKENGELLPI LRGEN