Recombinant human Crystallin zeta like 1/CRYZL1 protein

CRYZL1, also known as quinone oxidoreductase, is a protein that has sequence similarity to zeta crystalline. This Protein is present at low levels in human lens tissue. It has NADPH-dependent quinone reductase activity distinct from other known quinone reductases, and may play a role as a pH response element-binding protein. CRYZL1 is expressed at various levels in heart, brain, skeletal muscle, kidney, pancreas, liver and lung. Recombinant human CRYZL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Zeta crystallin homolog, Quinone reductase like 1, Quinone oxidoreductase-like protein 1, Quinone oxidoreductase like 1, QOH-1, 4P11

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

40.8 kDa (369aa) confirmed by MALDI-TOF

Additionnal Information

Zeta crystallin homolog, Quinone reductase like 1 protein, Quinone reductase like 1, Quinone oxidoreductase-like protein 1, Quinone oxidoreductase like 1, QOH-1, QOH1, QOH 1, CRYZL1, 4P11, ATGP0550-10 µg, ATGP0550-20 µg, ATGP0550-50 µg, ATGP0550-100 µg, ATGP0550-250 µg, ATGP0550-500 µg, ATGP0550-1 mg, ATGP0550-010, ATGP0550-020, ATGP0550-050, ATGP0550-100, ATGP0550-250, ATGP0550-500, ATGP0550-01M

References & Citations

Cai SF., et al. (2009) Molecules. 14 (8) :2935-46. ; ; Tauraite D., et al. (2009) Chem Phys Lipids. 159 (1) :45-50.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 0.1M NaCl, 20% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_665857

Uniprot Accession Number

O95825

Species

Human

AA Sequence

MKGLYFQQSS TDEEITFVFQ EKEDLPVTED NFVKLQVKAC ALSQINTKLL AEMKMKKDLF PVGREIAGIV LDVGSKVSFF QPDDEVVGIL PLDSEDPGLC EVVRVHEHYL VHKPEKVTWT EAAGSIRDGV RAYTALHYLS HLSPGKSVLI MDGASAFGTI AIQLAHHRGA KVISTACSLE DKQCLERFRP PIARVIDVSN GKVHVAESCL EETGGLGVDI VLDAGVRLYS KDDEPAVKLQ LLPHKHDIIT LLGVGGHWVT TEENLQLDPP DSHCLFLKGA TLAFLNDEVW NLSNVQQGKY LCILKDVMEK LSTGVFRPQL DEPIPLYEAK VSMEAVQKNQ GRKKQVVQF