Recombinant human GADD45 alpha protein
GADD45A, also known as growth arrest and DNA damage-inducible protein, binds both Cdks and PCNA, a protein involved in DNA replication and repair. It has been shown to stimulate DNA excision repair in vitro and to inhibit entry of cells into S phase. This protein may serve as a link between p53-dependent cell cycle checkpoint and DNA repair. Recombinant human GADD45A protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
19.4 kDa (173aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hollander MC. et al. (1993) J Biol Chem. 268 (32) :24385-93; ; Takekawa M. et al. (1998) Cell. 95 (4) :521-30.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl
Scientific Category
Cell Cycle
NCBI Accession Number
NP_001915
Uniprot Accession Number
P24522
Species
Human
Frequently Asked Questions
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