Recombinant human NIP7 protein
NIP7, also known as the 60S ribosome subunit biogenesis protein NIP7 homolog, belongs to NIP7 family and contains 1 PuA domain. It interacts with pre-ribosome complex and may bind to RNA. This protein is required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly. Recombinant human NIP7 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
21.5 kDa (188aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Sekiguchi T., et al. (2004) J. Biol. Chem. 279 (9) :8343-50.; ; Hesling C., et al. (2007) Exp. Cell. Res. 313 (20) :4180-95.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_057185
Uniprot Accession Number
Q9Y221
Species
Human
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