Recombinant human Calreticulin protein

Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, Calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Recombinant human Calreticulin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CC1qR, CRT, FLJ26680, RO, SSA, Autoantigen RO, CALR, CALR protein, Calregulin, Calreticulin, CRP55, CRTC, ERp60, FLJ26680, grp60, HACBP, Sicca syndrome antigen A.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

48.7 kDa (421aa) confirmed by MALDI-TOF

Additionnal Information

Calreticulin, cC1qR, CRT, FLJ26680, RO, SSA, Autoantigen RO, CALR, CALR protein, Calregulin, Calreticulin, CRP55, CRTC, ERp60, FLJ26680, grp60, HACBP, Sicca syndrome antigen A., ATGP0542-10 µg, ATGP0542-20 µg, ATGP0542-50 µg, ATGP0542-100 µg, ATGP0542-250 µg, ATGP0542-500 µg, ATGP0542-1 mg, ATGP0542-10, ATGP0542-20, ATGP0542-50, ATGP0542-100, ATGP0542-250, ATGP0542-500, ATGP0542-1

References & Citations

Kropp LE., et al. (2010) J Immunol. 184 (10) :5619-27. ; ; Singh P., et al. (2010) J Biochem. 147 (5) :625-32.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M Nacl, 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_004334.1

Uniprot Accession Number

P27797

Species

Human

AA Sequence

EPAVYFKEQ FLDGDGWTSR WIESKHKSDF GKFVLSSGKF YGDEEKDKGL QTSQDARFYA LSASFEPFSN KGQTLVVQFT VKHEQNIDCG GGYVKLFPNS LDQTDMHGDS EYNIMFGPDI CGPGTKKVHV IFNYKGKNVL INKDIRCKDD EFTHLYTLIV RPDNTYEVKI DNSQVESGSL EDDWDFLPPK KIKDPDASKP EDWDERAKID DPTDSKPEDW DKPEHIPDPD AKKPEDWDEE MDGEWEPPVI QNPEYKGEWK PRQIDNPDYK GTWIHPEIDN PEYSPDPSIY AYDNFGVLGL DLWQVKSGTI FDNFLITNDE AYAEEFGNET WGVTKAAEKQ MKDKQDEEQR LKEEEEDKKR KEEEEAEDKE DDEDKDEDEE DEEDKEEDEE EDVPGQAKDE L