Recombinant E.coli trxB protein

TRXB (Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E. coli TRXB protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 10 units/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.

Product Name Alternative

Thioredoxin reductase, Thioredoxin reductase JW0871, ECK0879, FAD/NAD (P) -binding, TRXR

Expression System

E.coli

Antigen Species

E.coli

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 10unit/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.

Molecular Weight

34.6 kDa (321aa) confirmed by MALDI-TOF

Additionnal Information

TRXB, Thioredoxin reductase, Thioredoxin reductase JW0871, ECK0879, Thioredoxin reductase, FAD/NAD (P) -binding, TRXR., ATGP0540-10 µg, ATGP0540-20 µg, ATGP0540-50 µg, ATGP0540-100 µg, ATGP0540-250 µg, ATGP0540-500 µg, ATGP0540-1 mg, ATGP0540-10, ATGP0540-20, ATGP0540-50, ATGP0540-100, ATGP0540-250, ATGP0540-500, ATGP0540-1

References & Citations

Faulkner MJ., et al. (2008) Proc Natl Acad Sci u S A. 105 (18) :6735-40. ; ; Takahata M., et al. (2008) J Biochem. 143 (4) :467-73.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

YP_003044110.1

Uniprot Accession Number

P0A9P4

Species

E.coli

AA Sequence

MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K