Recombinant mouse Sonic Hedgehog/Shh protein
SHH is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog (DHH) and Indian hedgehog (IHH) . This protien is the best studied ligand of the hedgehog signaling pathway. It plays a key role in regulating vertebrate organogenesis. SHH contain amino-terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions. Recombinant mouse SHH protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
20.8 kDa (183aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bak M., et al. (2004) Brain Res Mol Brain Res. 126 (2) :207-11. ; ; Dassule HR., et al. (2000) Development. 127 (22) :4775-85. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Development
NCBI Accession Number
NP_033196
Uniprot Accession Number
Q62226
Species
Mouse
AA Sequence
Frequently Asked Questions
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