Recombinant mouse Sonic Hedgehog/Shh protein

SHH is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog (DHH) and Indian hedgehog (IHH) . This protien is the best studied ligand of the hedgehog signaling pathway. It plays a key role in regulating vertebrate organogenesis. SHH contain amino-terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions. Recombinant mouse SHH protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Sonic hedgehog protein, Dsh, Hhg1, Hx, Hx13, Sonic hedgehog protein HHG 1, HHG1, HLP 3, HLP3, Holoprosencephaly 3, HPE 3, HPE3, MCOPCB5, SHH, SMMC I, SMMCI, Sonic Hedgehog (Drosophila) homolog, sonic hedgehog homolog (Drosophila), Sonic hedgehog homolog, TPT, TPTPS.

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

20.8 kDa (183aa) confirmed by MALDI-TOF

Additionnal Information

Sonic Hedgehog, Shh, Sonic hedgehog protein, Dsh, Hhg1, Hx, Hx13, Sonic hedgehog protein HHG 1, HHG1, HLP 3, HLP3, Holoprosencephaly 3, HPE 3, HPE3, MCOPCB5, SHH, SMMC I, SMMCI, Sonic Hedgehog (Drosophila) homolog, sonic hedgehog homolog (Drosophila), Sonic hedgehog homolog, TPT, TPTPS., ATGP0537-10 µg, ATGP0537-20 µg, ATGP0537-50 µg, ATGP0537-100 µg, ATGP0537-250 µg, ATGP0537-500 µg, ATGP0537-1 mg, ATGP0537-10, ATGP0537-20, ATGP0537-50, ATGP0537-100, ATGP0537-250, ATGP0537-500, ATGP0537-1

References & Citations

Bak M., et al. (2004) Brain Res Mol Brain Res. 126 (2) :207-11. ; ; Dassule HR., et al. (2000) Development. 127 (22) :4775-85. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Development

NCBI Accession Number

NP_033196

Uniprot Accession Number

Q62226

Species

Mouse

AA Sequence

MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKITRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGGLEHHH HHH